Title: Dysbiotic signatures and diagnostic potential of gut microbial markers for inflammatory bowel disease in Korean population

Journal: Scientific Reports

Authors: Hyun Sik Kim^1,8, Shin Ju Oh^2,8, Byung Kwon Kim^5,8, Ji Eun Kim², Bo-Hyung Kim^3,4,  Young-Kyu Park⁵, Bo-Gie Yang⁵, Jae-Yun Lee⁶, Jin-Woo Bae^1,6,7,* & Chang Kyun Lee^2,*

Affiliations

 ¹ Department of Biology, Kyung Hee University, Seoul, Republic of Korea

 ² Department of Gastroenterology, Center for Crohn’s and Colitis, Kyung Hee University Hospital, Kyung Hee University College of Medicine, Seoul, Republic of Korea

 ³ Department of Clinical Pharmacology and Therapeutics, Kyung Hee University Hospital, Seoul, Republic of Korea

 ⁴ East-West Medical Research Institute, Kyung Hee University, Seoul, Republic of Korea

 ⁵ Research Institute, GI Biome Inc., Seongnam, Republic of Korea

 ⁶ Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, Republic of Korea 

 ⁷ Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, Republic of Korea

 ⁸ Hyun Sik Kim, Shin Ju Oh and Byung Kwon Kim contributed equally to this work.

 ^* Correspondence

Abstract:

Fecal samples were collected from 640 individuals in Korea, including 523 patients with IBD (223 with Crohn’s disease [CD] and 300 with ulcerative colitis [UC]) and 117 healthy controls. The samples were subjected to cross-sectional gut metagenomic analysis using 16 S rRNA sequencing and bioinformatics analysis. Patients with IBD, particularly those with CD, exhibited significantly lower alpha diversities than the healthy subjects. Differential abundance analysis revealed dysbiotic signatures, characterized by an expansion of the genus Escherichia-Shigella in patients with CD. Functional annotations showed that functional pathways related to bacterial pathogenesis and production of hydrogen sulfide (H2S) were strongly upregulated in patients with CD. A dysbiosis score, calculated based on functional characteristics, highly correlated with disease severity. Markers distinguishing between healthy subjects and patients with IBD showed accurate classification based on a small number of microbial taxa, which may be used to diagnose ambiguous cases. These findings confirm the taxonomic and functional dysbiosis of the gut microbiota in patients with IBD, especially those with CD. Taxa indicative of dysbiosis may have significant implications for future clinical research on the management and diagnosis of IBD.

DOI: https://doi.org/10.1038/s41598-024-74002-6

Published: 10 October 2024

붙임:  Dysbiotic signatures and diagnostic potential of gut microbial markers for inflammatory bowel disease in Korean population.pdf